Jonathan David scored the only goal of the match in the first half.
Lille raised its score to 66 points to regain first place, while Paris Saint-Germain stuck at 63 points to fall to second place, only 7 rounds before the end of the competition.
The match saw a red card appearing in the face of Neymar Jr., as well as Thiago Djallo, a night player in the same shot.
Lille achieved his first victory over Paris Saint-Germain in the French League Princes’ Park since April 1996, that is, nearly a quarter of a century ago.
Lille woke up after losing the lead in the last round after suffering a sudden loss at home to Nim Olympique.
Paris Saint-Germain is preparing for a fierce clash against Bayern Munich next Wednesday night at the Allianz Arena by going into the Champions League quarter-finals, in a repeat of the final of the last version.
Saint-Germain coach Mauricio Pochettino was forced to push his best elements in order to overtake Lille and maintain the lead, so Neymar started next to Kylian Mbappe, Angel Di Maria and Moise Kane in the attacking line.
But the capital team ended the first half late with a goal by young Canadian David, after a pass by Jonathan Econdi.
In the second half, Saint-Germain tried to return to the match without success, and almost conceded a second deadly goal in the 80th minute from Turkish substitute Burak Yilmaz, but Keylor Navas shone.
The match was marked by 8 yellow cards from the referee, Benoit Bastian, and witnessed many violent interference.
Until the situation exploded in the 90th minute, when Neymar clashed with Dagalo, for both of them to obtain a second yellow card and leave the stadium expelled.
This is the third expulsion for Neymar in the last year and a half in the French League, more than any other player.